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Mass–energy equivalence states that all objects having mass, called massive objects, also have corresponding intrinsic energy, even when they are stationary. In the rest frame of an object, where by definition it is motionless and so has no momentum, the mass and energy are equivalent and they differ only by a constant, the speed of light squared. In Newtonian mechanics, a motionless body has no kinetic energy, and it may or may not have other amounts of internal stored energy, like chemical energy or thermal energy, in addition to any potential energy it may have from its position in a field of force. These energies tend to be much smaller than the mass of the object multiplied by the speed of light squared, which is on the order of 1019 Joules for a mass of one kilogram. Due to this principle, the mass of the atoms that come out of a nuclear reaction is less than the mass of the atoms that go in, and the difference in mass shows up as heat and light with the same equivalent energy as the difference. In analyzing these explosions, Einstein's formula can be used with E as the energy released and removed, and m as the change in mass.

In relativity, all the energy that moves with an object (i.e., the energy as measured in the object's rest frame) contributes to the total mass of the body, which measures how much it resists acceleration. If an isolated box of ideal mirrors could contain light, the individually massless photons would contribute to the total mass of the box, by the amount equal to their energy divided by c2. For an observer in the rest frame, removing energy is the same as removing mass and the formula m = E/c2 indicates how much mass is lost when energy is removed. In the same way, when any energy is added to an isolated system, the increase in the mass is equal to the added energy divided by c2.

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